FIBRINOLYTIC ACTIVITY IN HEART TISSUE IN HYPOTHYROID AND HYPERTHYROID RATS UNDER THE INFLUENCE OF EXOGENOUS MELATONIN AND BLINDING CONDITIONS (ENUCLEATION)

Svitlana Anokchina; Olena Yasinska

doi:10.23856/4626

Svitlana Anokchina Bukovinian State Medical University http://orcid.org/0000-0002-6808-9916
Olena Yasinska Bukovinian State Medical University http://orcid.org/0000-0002-7389-0804

Słowa kluczowe: exogenous melatonin, fibrinolytic activity, hypothyroidism, hyperthyroidism, enucleation, heart tissues

Abstrakt

The aim of our scientific work was to study the effect of exogenous melatonin on the indicators of fibrinolytic processes in the heart tissues and to analyze the changes in fibrinolytic activity occurring in the heart tissues of enucleated hyper- and hypothyroid white rats. The experiments were carried out on white nonlinear male rats weighing 0.12-0.14 kg. 4 experimental groups of animals were formed. The control group consisted of 11 normothyroid rats, which were injected with a melatonin solution in appropriate volumes. The animals were euthanized under light ether anesthesia. Samples of the examined heart tissue were ground in a glass homogenizer with borate buffer (pH 9.0). The homogenate was used in biochemical analysis. Determination of the total, enzymatic and non-enzymatic fibrinolytic activity (TFA, EFA, NFA, respectively) in the heart tissues was carried out by the method of azofibrin lysis (LLC "Simko", Ukraine). Statistical processing of the results was carried out by the method of variation statistics using the Student's test. Experimental studies on nonlinear male white rats have shown that the introduction of exogenous melatonin, as well as modeling the conditions of endogenous melatonin overproduction by blinding (enucleation) cause an increase in the intensity of enzymatic and non-enzymatic fibrinolysis in heart tissue in normotyroid animals. At other hand, enucleation causes an increase in the intensity of fibrinolysis in the tissues of the heart in hypothyroid rats, but not in hyperthyroid.

Wykaz bibliografii

1. Anokhina, S.I. (2002). Kharakterystyka zmin koahuliatsiinoho potentsialu, fibrynolitychnoi aktyvnosti plazmy krovi ta tkanyn vnutrishnikh orhaniv v osliplenykh shchuriv [Characteristics of changes in coagulation potential, fibrinolytic activity of blood plasma and tissues of internal organs in blinded rats]. Bukovynskyi medychnyi visnyk [Bukovinian Medical Herald], Vol. 6, No 4, 168-171. [in Ukrainian]
2. Anokhina, S.I. (2004). Rol shyshkopodibnoho tila u rehuliatsii hemostazu pry hipo- ta hipertyreoidnykh stanakh [The role of pineal gland in haemostasis regulation attached to hypoand hyperthyreoid status] Avtoref. dys. … k. med. nauk [Abstract of the dissertation of the candidate of medical sciences]:14.03.04. Ternopil. [in Ukrainian]
3. Anysymov, V.N. (2006). Melatonin i ego mesto v sovremennoj medicine. [Melatonin and its place in modern medicine]. Russkij medicinskij zhurnal [Russian medical journal], No 14, 4, 269-273. (in Russian)
4. Arushanyan, E.B., Shetinin, E.V. (2016) Melatonin kak universalnyj modulyator lyubyh patologicheskih processov [Melatonin as a universal modulator of any pathological processes] Patologicheskaya fiziologiya i eksperimentalnaya terapiya [Pathological physiology and experimental therapy], No 1,79-88. (in Russian)
5. Garcia-Marin, R., Fernandez-Santos, J.M., Morillo-Bernal. J., Gordillo-Martinez, F., Vazquez-Roman, V., Utrilla, J.C., Carrillo-Vico, A., Guerrero, J.M., Martin-Lacave, I. (2015). Melatonin in the thyroid gland: regulation by thyroid-stimulating hormone and role in thyroglobulin gene expression. J Physiol Pharmacol. 66(5),643-52.
6. Komarov, F.I., Rapoport, S.I. (2000). Hronobiologiya i hronomedicina [Chronobiology and chronomedicine]. Moscow:Triada-H. (in Russian)
7. Kuchuk, O.P. (2001). Patohenetychni osoblyvosti zapalnoho protsesu pry pronyknykh poranenniakh zadnoho sehmenta oka i profilaktyka pisliatravmatychnykh uskladnen [Pathogenetic features of the inflammatory process in penetrating injuries of the posterior segment of the eye and prevention of post-traumatic complications]: Avtoref. dys. na zdobuttia nauk. stupenia kand. med. nauk [Abstract of the dissertation of the candidate of medical sciences]: 14.03.04. Ternopil, 17. [in Ukrainian]
8. Kukharchuk, O.L. (1996). Patohenetychna rol ta metody korektsii intehratyvnykh porushen hormonalno-mesendzhernykh system rehuliatsii homeostazu natriiu pry patolohii nyrok [Pathogenetic role and methods of correction of integrative disorders of hormonal-messenger systems of regulation of sodium homeostasis in renal pathology]: Avtoref. dys. … d-ra med. nauk [Abstract of the dissertation of the doctor of medical sciences]: 14.03.05. Odesa. [in Ukrainian]
9. Lin, J.D., Fang, W.F., Tang, K.T. et al. (2019). Effects of exogenous melatonin on clinical and pathological features of a human thyroglobulin-induced experimental autoimmune thyroiditis mouse model. Sci Rep 9, 5886. https://doi.org/10.1038/s41598-019-42442-0
10. Litvinov, R. I. (2013). Molekulyarnye mehanizmy i klinicheskoe znachenie fibrinoliza [Molecular mechanisms and clinical significance of fibrinolysis]. Казанский медицинский журнал [Kazan Medical Journal], vol. 94, No 5, 711-718. (in Russian)
11. Ma, Wy., Song, Rj., Xu, Bb. et al. (2021). Melatonin promotes cardiomyocyte proliferation and heart repair in mice with myocardial infarction via miR-143-3p/Yap/Ctnnd1 signaling pathway. Acta Pharmacol Sin,42, 921-931. https://doi.org/10.1038/s41401-020-0495-2.
12. Ostrowska, Z., Kos-Kudła, B., Marek, B. [et al.] (2004). Circadian rhythm of melatonin in рatients with hyрertension. Pol Merkur Lekarski, vol.17, No 97, 50-54.
13. Perepeliuk, M.D. (1992). Kyslotovydilna funktsiia nyrok pry eksperymentalnomu hipertyreozi [Acid-excretory function of the kidneys in experimental hyperthyroidism]: Avtoref. dys. Na zdobuttia nauk. stupenia kand. med. nauk [Abstract of the dissertation of the candidate of medical sciences]: 14.03.04, Lviv. [in Ukrainian]
14. Tan, D.X., Hardeland, R., Manchester, L.C., Paredes, S.D., Korkmaz, A., Sainz, R.M., Mayo, J.C., Fuentes-Broto, L., Reiter, R.J. (2010). The changing biological roles of melatonin during evolution: from an antioxidant to signals of darkness, sexual selection and fitness. Biol Rev Camb Philos Soc, 85, 607-623.
15. Violi, F., Ferro, D. (2013). Clotting activation and hyрerfibrinolysis in cirrhosis: imрlication for bleeding and thrombosis. Seminars Thrombosis and Hemostasis, 39(04), 426-433.
16. Zaslavskaya, R.M., Shakirova, A.N., Lilica, G.V., Sherban, E.A. (2005). Melatonin v kompleksnom lechenii bolnyh serdechno-sosudistymi zabolevaniyami [Melatonin in the complex treatment of patients with cardiovascular diseases]. Moscow: ID MEDPRAKTIKA-M (in Russian)
17. Zhao Dake, Yu Yang, Shen Yong, Liu Qin, Zhao Zhiwei, Sharma Ramaswamy, Reiter Russel J. (2019). Melatonin Synthesis and Function: Evolutionary History in Animals and Plants. Frontiers in Endocrinology, Vol.10, 249. https://doi.org/10.3389/fendo.2019.00249