EFFECT OF ACUTE INTOXICATION WITH AFLATOXIN B1 ON THE ANTIOXIDANT SYSTEM IN RED BLOOD CELLS AND LUNG CELLS OF WHITE RATS
Abstrakt
Contamination of agricultural products with mycotoxins is a widespread phenomenon that is manifested in many countries. Among mycotoxins, which significantly affect human and animal health, aflatoxins produced by Aspergillus micromycetic fungi (mainly A. flavus) are of serious concern because of their high toxicity and carcinogenicity. The aim of our study was to investigate the effect of aflatoxin B1 (AFB1) on intracellular metabolism in laboratory animals. The dynamics of the lipid peroxidation (LPO) and activities of antioxidant system enzymes (superoxide dismutase, glutathione peroxidase, and glutathione reductase) in lung cells and erythrocytes of white rats were analyzed within 14 days after administration of AFB1 (dose 1/10 LD50). The results of the study have shown the activation of the LPO processes and a decrease in the activity of enzymes of the antioxidant system in the cells of rats of the experimental groups. In general, the obtained results suggest the development of oxidative stress in the lungs cells and erythrocytes of animals under the influence of aflatoxin B1.
Wykaz bibliografii
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Antonyak, H., Olijnyk, Ch., Koval, N., Fedyakov, R., Dosviadchynska, M, Panchuk, I., Chekh, B. (2015). Effects of aflatoxin B1 on lipid peroxidation and activities of antioxidant enzymes in rat organs and erythrocytes. Visnyk Lvivskoho Universytetu Seriia Biolohichna, 69, 41–48. [In Ukrainian].
Arthur, J. R. (2000). The glutathione peroxidases. Cellular and Molecular Life Sciences, 57, 1825–1835. [in English].
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Dagnas, S., Membre, J.-M. (2013). Predicting and preventing mold spoilage of food products. J. Food Protection, 76 (3), 538–551. DOI:10.4315/0362-028X.JFP-12-349. [in English].
D’Angelo, A., Bellino, C., Alborali G. L. et al. (2007). Neurological signs associated with aflatoxicosis in Piedmontese calves. Veterinary Record, 160(20), 698-700. [in English].
Du, X., Schrunk, D. E., Imerman, P. M., Smith L, Francis, K., Tahara, J., Tkachenko, A., Reimschuessel, R., Rumbeiha, W. K. (2019). Evaluation of a diagnostic method to quantify aflatoxins B1 and M1 in animal liver by high-performance liquid chromatography with fluorescence detection. The Journal of AOAC International. Doi: 10.5740/jaoacint.18-0355. [in English].
El-Beltagi, H. S., Mohamed, H. I. (2013). Reactive oxygen species, lipid peroxidation and antioxidative defense mechanism. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(1), 44–57. DOI: 10.15835/nbha4118929. [in English].
Eraslan, G., Sarıca, Z. S., Bayram, L. Ç., Tekeli, M. Y., Kanbur, M., Karabacak, M. (2017). The effects of diosmin on aflatoxin-induced liver and kidney damage. Environmental science and pollution research international, 24(36), 27931-27941. DOI: 10.1007/s11356-017-0232-7. [in English].
Fang, L., Tian, M., Yan, X., Xiao, W. (2018). Isolation of aflatoxin b1 from moldy foods by solid-phase extraction combined with bifunctional ionic liquid-based silicas. Journal of Analytical Methods in Chemistry, vol. 2018, Article ID 8427580. DOI: 10.1155/2018/8427580. [in English].
Silva, J. C., Minto, R. E., Barry III, C. E., Holland, K. A., Townsend, C. A. (1996). Isolation and Characterization of the Versicolorin B Synthase Gene from Aspergillus parasiticus: expansion of the aflatoxin b1 biosynthetic gene cluster. The Journal of Biological Chemistry, 271, 13600-13608. DOI:10.1074/jbc.271.23.13600. [in English].
Knipstein, B., Huang, J., Barr, E., Sossenheimer, P., Dietzen, D., Egner, P. A., Groopman, J. D., Rudnick, D. A. (2015). Dietary aflatoxin-induced stunting in a novel rat model: evidence for toxin-induced liver injury and hepatic growth hormone resistance. Pediatric Research, 78(2), 120-127. DOI: 10.1038/pr.2015.84. [in English].
Khlangwiset, P., Shephard, G. S., Wu, F. (2011). Aflatoxins and growth impairment: a review. Critical Reviews in Toxicology, 41(9), 740-755. [in English].
Lapach, S. N., Chubenko, A. V., Babich, P. N. (2001). Statistical methods in medical-biological research using Excel. Moscow: Morion, 408 p. [ іn Russian].
Li, Y., Ma, Q. G., Zhao, L. H., Guo, Y. Q., Duan, G. X., Zhang, J. Y., & Ji, C. (2014). Protective efficacy of alpha-lipoic acid against aflatoxin B1-induced oxidative damage in the liver. Asian-Australasian Journal of Animal Sciences, 27(6), 907– 915. [in English].
Prokhorov, M. I. (1982). Methods of biochemical research. Leningrad: Publishing House of the Leningrad University. [іn Russian].
Rotimi, O. A., Rotimi, S. O., Oluwafemi, F., Ademuyiwa, O., Balogun, E. A. (2018). Oxidative stress in extrahepatic tissues of rats co-exposed to aflatoxin b1 and low protein diet. Toxicological Research, 34(3), 211-220. DOI: 10.5487/TR.2018.34.3.211. [in English].
Skládanka, J., Nedělník, J., Adam, V., Doležal, P., Moravcová, H., Dohnal, V. (2011). Forage as a primary source of mycotoxins in animal diets. The International Journal of Environmental Research and Public Health, 8(1): 37–50. DOI: 10.3390/ijerph8010037. [in English].
Sobral, M. M. C, Faria, M. A., Cunha, S. C., Ferreira, IM. (2018). Toxicological interactions between mycotoxins from ubiquitous fungi: Impact on hepatic and intestinal human epithelial cells. Chemosphere, 202, 538-548. DOI: 10.1016/j.chemosphere.2018.03.122. [in English].
Souto, N. S., Claudia Monteiro Braga, A., Lutchemeyer de Freitas, M., Rechia Fighera, M., Royes, L. F. F., Schneider Oliveira, M., Furian, A. F. (2018). Aflatoxin B1 reduces non-enzymatic antioxidant defenses and increases protein kinase C activation in the cerebral cortex of young rats. Nutritional Neuroscience, 21(4), 268-275. DOI: 10.1080/1028415X.2017.1278837. [in English].
Thomas, E., Willia, M., Jagdish, K., at al. (1996). Binding of the aflatoxin-glutathione conjugate to mouse glutathione S-transferase A3-3 is saturated at only one ligand per dimer. The Journal of Biological Chemistry, 271, 27470-27474. DOI:10.1074/jbc.271.44.27470. [in English].
Wang, J., Tang, L., Glenn, T. C., Wang, J. S. (2016). Aflatoxin B1 Induced Compositional Changes in Gut Microbial Communities of Male F344 Rats. Toxicological sciences, 150 (1), 54-63. DOI:10.1093/toxsci/kfv259. [in English].
Antonyak, H. L., Kalynets-Mamchur, Z. I., Dudka, I. O., Babych, N. O., Panas, N. E. (2013). Ecology of Fungi (monograph). Lviv: Ivan Franko National University of Lviv (Series “Biological Studies”). [In Ukrainian].
Antonyak, H., Olijnyk, Ch., Koval, N., Fedyakov, R., Dosviadchynska, M, Panchuk, I., Chekh, B. (2015). Effects of aflatoxin B1 on lipid peroxidation and activities of antioxidant enzymes in rat organs and erythrocytes. Visnyk Lvivskoho Universytetu Seriia Biolohichna, 69, 41–48. [In Ukrainian].
Arthur, J. R. (2000). The glutathione peroxidases. Cellular and Molecular Life Sciences, 57, 1825–1835. [in English].
Bennett, J. W., Klich, M. (2003). Mycotoxins. Clinical Microbiology Reviews, 16(3), 497–516. DOI: 10.1128/CMR.16.3.497-516.2003. [in English].
Dagnas, S., Membre, J.-M. (2013). Predicting and preventing mold spoilage of food products. J. Food Protection, 76 (3), 538–551. DOI:10.4315/0362-028X.JFP-12-349. [in English].
D’Angelo, A., Bellino, C., Alborali G. L. et al. (2007). Neurological signs associated with aflatoxicosis in Piedmontese calves. Veterinary Record, 160(20), 698-700. [in English].
Du, X., Schrunk, D. E., Imerman, P. M., Smith L, Francis, K., Tahara, J., Tkachenko, A., Reimschuessel, R., Rumbeiha, W. K. (2019). Evaluation of a diagnostic method to quantify aflatoxins B1 and M1 in animal liver by high-performance liquid chromatography with fluorescence detection. The Journal of AOAC International. Doi: 10.5740/jaoacint.18-0355. [in English].
El-Beltagi, H. S., Mohamed, H. I. (2013). Reactive oxygen species, lipid peroxidation and antioxidative defense mechanism. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(1), 44–57. DOI: 10.15835/nbha4118929. [in English].
Eraslan, G., Sarıca, Z. S., Bayram, L. Ç., Tekeli, M. Y., Kanbur, M., Karabacak, M. (2017). The effects of diosmin on aflatoxin-induced liver and kidney damage. Environmental science and pollution research international, 24(36), 27931-27941. DOI: 10.1007/s11356-017-0232-7. [in English].
Fang, L., Tian, M., Yan, X., Xiao, W. (2018). Isolation of aflatoxin b1 from moldy foods by solid-phase extraction combined with bifunctional ionic liquid-based silicas. Journal of Analytical Methods in Chemistry, vol. 2018, Article ID 8427580. DOI: 10.1155/2018/8427580. [in English].
Silva, J. C., Minto, R. E., Barry III, C. E., Holland, K. A., Townsend, C. A. (1996). Isolation and Characterization of the Versicolorin B Synthase Gene from Aspergillus parasiticus: expansion of the aflatoxin b1 biosynthetic gene cluster. The Journal of Biological Chemistry, 271, 13600-13608. DOI:10.1074/jbc.271.23.13600. [in English].
Knipstein, B., Huang, J., Barr, E., Sossenheimer, P., Dietzen, D., Egner, P. A., Groopman, J. D., Rudnick, D. A. (2015). Dietary aflatoxin-induced stunting in a novel rat model: evidence for toxin-induced liver injury and hepatic growth hormone resistance. Pediatric Research, 78(2), 120-127. DOI: 10.1038/pr.2015.84. [in English].
Khlangwiset, P., Shephard, G. S., Wu, F. (2011). Aflatoxins and growth impairment: a review. Critical Reviews in Toxicology, 41(9), 740-755. [in English].
Lapach, S. N., Chubenko, A. V., Babich, P. N. (2001). Statistical methods in medical-biological research using Excel. Moscow: Morion, 408 p. [ іn Russian].
Li, Y., Ma, Q. G., Zhao, L. H., Guo, Y. Q., Duan, G. X., Zhang, J. Y., & Ji, C. (2014). Protective efficacy of alpha-lipoic acid against aflatoxin B1-induced oxidative damage in the liver. Asian-Australasian Journal of Animal Sciences, 27(6), 907– 915. [in English].
Prokhorov, M. I. (1982). Methods of biochemical research. Leningrad: Publishing House of the Leningrad University. [іn Russian].
Rotimi, O. A., Rotimi, S. O., Oluwafemi, F., Ademuyiwa, O., Balogun, E. A. (2018). Oxidative stress in extrahepatic tissues of rats co-exposed to aflatoxin b1 and low protein diet. Toxicological Research, 34(3), 211-220. DOI: 10.5487/TR.2018.34.3.211. [in English].
Skládanka, J., Nedělník, J., Adam, V., Doležal, P., Moravcová, H., Dohnal, V. (2011). Forage as a primary source of mycotoxins in animal diets. The International Journal of Environmental Research and Public Health, 8(1): 37–50. DOI: 10.3390/ijerph8010037. [in English].
Sobral, M. M. C, Faria, M. A., Cunha, S. C., Ferreira, IM. (2018). Toxicological interactions between mycotoxins from ubiquitous fungi: Impact on hepatic and intestinal human epithelial cells. Chemosphere, 202, 538-548. DOI: 10.1016/j.chemosphere.2018.03.122. [in English].
Souto, N. S., Claudia Monteiro Braga, A., Lutchemeyer de Freitas, M., Rechia Fighera, M., Royes, L. F. F., Schneider Oliveira, M., Furian, A. F. (2018). Aflatoxin B1 reduces non-enzymatic antioxidant defenses and increases protein kinase C activation in the cerebral cortex of young rats. Nutritional Neuroscience, 21(4), 268-275. DOI: 10.1080/1028415X.2017.1278837. [in English].
Thomas, E., Willia, M., Jagdish, K., at al. (1996). Binding of the aflatoxin-glutathione conjugate to mouse glutathione S-transferase A3-3 is saturated at only one ligand per dimer. The Journal of Biological Chemistry, 271, 27470-27474. DOI:10.1074/jbc.271.44.27470. [in English].
Wang, J., Tang, L., Glenn, T. C., Wang, J. S. (2016). Aflatoxin B1 Induced Compositional Changes in Gut Microbial Communities of Male F344 Rats. Toxicological sciences, 150 (1), 54-63. DOI:10.1093/toxsci/kfv259. [in English].
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Opublikowane
2019-04-03
Jak cytować
Dosviadchynska, M., & Antonyak, H. (2019). EFFECT OF ACUTE INTOXICATION WITH AFLATOXIN B1 ON THE ANTIOXIDANT SYSTEM IN RED BLOOD CELLS AND LUNG CELLS OF WHITE RATS. Periodyk Naukowy Akademii Polonijnej, 34(3), 84-90. https://doi.org/10.23856/3410
Dział
Nauki Medyczne
